« Previous Article
Next Article »

Original Research Open Access


The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors

Sahar K. Alzubaidy.

Cited by (1)

Abstract
The aim of this study is the evaluation the resistance of S. marcescence obtained from soil and water to metals chlorides (Zn+2, Hg+2, Fe+2, Al+3, and Pb+2). Four isolates, identified as Serratia marcescence and S. marcescena (S4) were selected for this study according to their resistance to five heavy metals. The ability of S. marcescena (S4) to grow in different concentrations of metals chloride (200-1200 μg/ml) was tested; the highest concentration that S. marcescence (S4) tolerate was 1000 μg/ml for Zn+2, Hg+2, Fe+2, AL+3, pb+2 and 300 μg/ml for Hg+2 through 24 hrs incubation at 37 Co. The effects of temperature and pH on bacteria growth during 72 hrs were also studied. S. marcescence (S4) was affected by ZnCl2, PbCl2, FeCl2, and AlCl3 during 24 hrs, while mercury causes no bacterial growth. S. marcescence (S4) showed growth in temperature range of 30-50 Co in presence of 4 metals. The isolates showed the ability to grow in different pH values (4, 7 and 9) in presence of four metals in all pH values (1000 μg/ml) and un-ability to grow with 300 μg/ml Hg+2. The highest Zn+2 removal ratio was 75% then Pb+2 55% while Fe+2 has the lowest removal ratio (48%). The study was conducted in the central lab of College of sciences/University of Baghdad/Iraq in 2011-2012. It was conclude that the identified heavy metal resistant bacteria could be useful for bioremediation of heavy metals in the contaminated soil and water.

Key words: Resistance, Serratia marcescence, Heavy metals chlorides


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Sahar K. Alzubaidy
on Google
on Google Scholar
Article Statistics
 Viewed: 2918
Downloaded: 813
Cited: 1

REFERENCES
1- Blessing B, Sussmuth R. Influence of combined chemicals on prodigiosin synthesis of Serratia marcescence. Environ Toxicol Water Quality 1991; 6(4); 371-382.
http://dx.doi.org/10.1002/tox.2530060403


2- Sa'id M. Experimental studies on effect of high metals presence in industrial waste water on biological treatment. Indust J Environ Sci 2010; 1(4): 666-676.


3- Nies DH. Microbial heavy-metal resistance. Appl Microbial Biotechnol 1999; 51(16):730-750.
http://dx.doi.org/10.1007/s002530051457
[
Pubmed]   

4- David N, Benjamin L, Patrick O. Some physico-chemical and heavy metal levels in soils of waste dumpsites in Port Harcourt Municipality and Environs. J Appl Sci Environ Manage 2009; 13(4):65-70.


5- Moore JW, Ramamoorthy S. Impact of heavy metals in natural waters. Heavy metals in natural waters: Applied monitoring and impact assessment, Springer-Verlag; 1984.p. 205-233.


6- Connell DW, Miller GJ. Chemistry and Ecotoxicology of Pollution, John Wiley & Sons, NY, 1984; p.444.


7- Hetzer A, Daughngy CJ, Morgan HW. Cadmium ion biosorption by thermophilic bacteria Geobacillus stearothermophilus and G thermocatenulatus. Appl Environ Microbiol 2006; 72:4020-4027.
http://dx.doi.org/10.1128/AEM.00295-06
[
Pubmed]    [PMC Free Fulltext]   

8- Karbasizaed V, Badami N, Emtiazi G. Antimicrobial heavy metal resistance and plasmid profile of cdiforms isolated from nosocomal African. Afr J Biotechnol 2003; 2(10): 379-383.


9- Haddix P, Werner TF. Spectrophtometric assay of gene expression Serratia marcescence pigmentation. Biosceine 2000; 26(4):3-12.


10- Holt JG, Krieg NR, Sneath HA, Stalely JT, Wiliams ST. Berge's Manual of Determinative Bacteriology, 9th edition, Williams and wilkins; 1994.


11- Rajbanshi A. Study on heavy metal resistant bacteria in guheswori sewage treatment plant. Our Nature. 2008; 6:52-57.


12- Vandepitte J, Verhaegen J, Engbaek K, Rohnor P, Piot P, Heuck CC. Basic laboratory procedures in clinical bacteriology. 2nd edition. World Health Organization; 2003.


13- Sundar K, Vidya R, Mukherjee A, Chandrasekaran N. High chromium tolerant bacterial strains from Palar River Basin: Impact of tannery pollution. Res J Environ Earth Sci 2010; 2(2):112-117.


14- Abdelatey LM, Khalil WKB, Ali TH, Mahrous KF. Heavy metal resistance and gene expression analysis of metal resistance genes in gram-positive and gram-negative bacteria present in Egyptian soils. J Appl Sci Environ Sanitation 2011; 6(2): 201-211.


15- Philip L, Lyengar L, Venkobachar C. Biosorption of U, La, Pr, Nd, Eu and Dy by Pseudomonas aeruginosa. J Indust Microbiol Biotechnol 2000; 25:1-7.
http://dx.doi.org/10.1038/sj.jim.7000026


16- Qin Y, Shi B, Liu J. Application of chitosan and alginate in treating waste water containing heavy metal ions. Indian J Chem Technol 2006; 13:464-469.


17- Sethuraman P, Dharmendirakumar M. Application of bacteria to remove Ni(II) Irons from aqueous solution. Eur J Sci Res 2011; 52(3):345-358.


18- Silver S. Bacterial resistances to toxic metal ions. Gene 1996; 179:9-19.
http://dx.doi.org/10.1016/S0378-1119(96)00323-X


19- Wuertz S, Mergeay M. The impact of heavy metals on soil microbial communities and their activities. In: van Elsas JD, Wellington EMH, Trevors JT, (Eds.), Modern Soil Microbiology. Marcel Decker, NY, 1997; 1-20.


20- Raja EE, Anbazhagan C, Kiand Selvam GS. Isolation and characterization of metal resistant Pseudomonas aeruginosa strain. World J Microbiol Biotechnol 2006; 22:577-589.
http://dx.doi.org/10.1007/s11274-005-9074-4


21- Wagner-Dobler I, Lunsdorf H, Lubbehusen T, Von Canstein HF, Li Y. Structure and species composition of mercury-reducing biofilms. Appl Environ Microbiol 2000; 66:4559-4569.
http://dx.doi.org/10.1128/AEM.66.10.4559-4563.2000
[
Pubmed]    [PMC Free Fulltext]   

22- Sarhan OA. Study of biosorption of some heavy metals by local isolates of Zoogloea ramigera. MSc Thesis, College of Science, Baghdad, 2004.


23- Erum S. Genetic basis of heavy metal tolerance in bacteria. PhD thesis, University of Karachi, Karachi, 2006.


24- Congeevaram SH, Dhanarani S, Park J, Dexilin M, Thamaraiselvi K. Biosorption of chromium and nickel by heavy metal resistant fungal and bacterial isolates. J Hazard Materials 2007; 146:270-277.
http://dx.doi.org/10.1016/j.jhazmat.2006.12.017
[
Pubmed]   

25- Burke BE, Pfister RM. Cadmium transport by a Cd+2- sensitive and a Cd+2-resistant strain of Bacillus subtilis. Can J Microbiol 1986; 32:539-542.
http://dx.doi.org/10.1139/m86-100
[
Pubmed]   

26- Mullen MD, Wolf DC, Ferris FG, Beveridge TJ, Flemming CA, Bailey GW. Bacterial sorption of heavy metals. Appl Environ Microbiol 1989; 55: 3143-3149.
[
Pubmed]    [PMC Free Fulltext]   

27- Wei Y, Chen W. Enhanced of production of prodigiosin-like pigment from Serratia marcescens SMΔR by medium improvement and oil supplementation strategies. J Biosci Bioengin 2005; 99(6):616-622.
http://dx.doi.org/10.1263/jbb.99.616
[
Pubmed]   

This Article Cited By the following articles

Biodegradation of cyanide and evaluation of kinetic models by immobilized cells of Serratia marcescens strain AQ07
Int. J. Environ. Sci. Technol. 2017; (): .

1
 

How to Cite this Article
Pubmed Style

Sahar K. Alzubaidy. The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. J Environ Occup Sci. 2012; 1(1): 37-42. doi:10.5455/jeos.20120605104508



Web Style

Sahar K. Alzubaidy. The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. www.scopemed.org/?mno=20669 [Access: August 17, 2017]. doi:10.5455/jeos.20120605104508



AMA (American Medical Association) Style

Sahar K. Alzubaidy. The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. J Environ Occup Sci. 2012; 1(1): 37-42. doi:10.5455/jeos.20120605104508



Vancouver/ICMJE Style

Sahar K. Alzubaidy. The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. J Environ Occup Sci. (2012), [cited August 17, 2017]; 1(1): 37-42. doi:10.5455/jeos.20120605104508



Harvard Style

Sahar K. Alzubaidy (2012) The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. J Environ Occup Sci, 1 (1), 37-42. doi:10.5455/jeos.20120605104508



Turabian Style

Sahar K. Alzubaidy. 2012. The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. Journal of Environmental and Occupational Science, 1 (1), 37-42. doi:10.5455/jeos.20120605104508



Chicago Style

Sahar K. Alzubaidy. "The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors." Journal of Environmental and Occupational Science 1 (2012), 37-42. doi:10.5455/jeos.20120605104508



MLA (The Modern Language Association) Style

Sahar K. Alzubaidy. "The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors." Journal of Environmental and Occupational Science 1.1 (2012), 37-42. Print. doi:10.5455/jeos.20120605104508



APA (American Psychological Association) Style

Sahar K. Alzubaidy (2012) The resistance of locally isolated Serratia marcescens to heavy metals chlorides and optimization of some environmental factors. Journal of Environmental and Occupational Science, 1 (1), 37-42. doi:10.5455/jeos.20120605104508